Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Document date: 2017_9_21
ID: 1k9l0z5k_27
Snippet: The Furthermore, the temperature of the RT-LAMP reaction was determined by incubating the reaction systems at 60, 61, 62, 63, and 64 °C for 70 min in a water bath, respectively, and then the reactions were terminated by heating up at 80 °C for 10 min. The RT-LAMP products were electrophoresed on 2.0% agarose gel in 1× TAE buffer, or directly visualized by adding 1× SYBR green I in the reaction system for diction by a color change. In addition.....
Document: The Furthermore, the temperature of the RT-LAMP reaction was determined by incubating the reaction systems at 60, 61, 62, 63, and 64 °C for 70 min in a water bath, respectively, and then the reactions were terminated by heating up at 80 °C for 10 min. The RT-LAMP products were electrophoresed on 2.0% agarose gel in 1× TAE buffer, or directly visualized by adding 1× SYBR green I in the reaction system for diction by a color change. In addition, the RT-LAMP products were identified by the digestion of SmlI (New England Biolabs, USA).
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