Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Document date: 2017_9_21
ID: 1k9l0z5k_33
Snippet: A total of 192 fecal/intestinal clinical samples of piglets with severe watery diarrhea were collected from 35 pig farms in Jiangxi, China during 2014 and 2015. All samples were then selected to detect the presence of PDCoV by conventional RT-PCR, nested RT-PCR, and RT-LAMP. The samples were resuspended in saline, subsequently vortexed and then centrifuged at 8000×g for 5 min at 4 °C. The supernatants were harvested and stored at −80 °C unti.....
Document: A total of 192 fecal/intestinal clinical samples of piglets with severe watery diarrhea were collected from 35 pig farms in Jiangxi, China during 2014 and 2015. All samples were then selected to detect the presence of PDCoV by conventional RT-PCR, nested RT-PCR, and RT-LAMP. The samples were resuspended in saline, subsequently vortexed and then centrifuged at 8000×g for 5 min at 4 °C. The supernatants were harvested and stored at −80 °C until use. Viral RNA was extracted and used as a template for conventional RT-PCR, nested RT-PCR, and RT-LAMP according to aforementioned methods.
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