Author: Yichun Wang; Usha Kadiyala; Zhibei Qu; Paolo Elvati; Christopher Altheim; Nicholas A. Kotov; Angela Violi; J. Scott VanEpps
Title: Anti-biofilm Activity of Graphene Quantum Dots via Self-Assembly with Bacterial Amyloid Proteins Document date: 2019_2_19
ID: e0sxynb1_40
Snippet: Biofilm ECM Isolation. Biofilm ECM was harvested by disrupting biofilms using vortex and sonication as previously described. 32 Briefly fibers were collected after 5-day static growth by scraping biofilms into 3 mL of potassium phosphate buffer (50 mM, pH 7.4). The biofilm suspensions were homogenized using a tissue homogenizer (Power 4, 3 minutes) to shear fibers free from the cell walls. Supernatants were clarified by repeated (×2) centrifugat.....
Document: Biofilm ECM Isolation. Biofilm ECM was harvested by disrupting biofilms using vortex and sonication as previously described. 32 Briefly fibers were collected after 5-day static growth by scraping biofilms into 3 mL of potassium phosphate buffer (50 mM, pH 7.4). The biofilm suspensions were homogenized using a tissue homogenizer (Power 4, 3 minutes) to shear fibers free from the cell walls. Supernatants were clarified by repeated (×2) centrifugation at 4,000 g for 15 minutes to remove cells. The cell-free supernatant was incubated in 200 mM NaCl for 24 hours at room temperature. The fibers were isolated using Millipore Amicon Ultra Centrifugal Filter Units with a pore size of 30 kDa.
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