Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens Document date: 2016_8_9
ID: 16e99fuz_50
Snippet: β6 Integrin Enhances Lung Injury during Influenza Lung preparation. Lungs were finely minced with a surgical blade and digested in Neurobasal medium (Gibco) containing 1 mg/ml DNaseI (Worthington), 2.5 mg/ml Collagenase P (Roche), 2.5 mg/ml Collagenase/Dispase (Roche), and 1x B27 and N2 supplements (Gibco) at 37°C for 30 min. The digest was dissociated though a 1 ml pipette tip and passed through a 70 μM nylon mesh to obtain a single cell susp.....
Document: β6 Integrin Enhances Lung Injury during Influenza Lung preparation. Lungs were finely minced with a surgical blade and digested in Neurobasal medium (Gibco) containing 1 mg/ml DNaseI (Worthington), 2.5 mg/ml Collagenase P (Roche), 2.5 mg/ml Collagenase/Dispase (Roche), and 1x B27 and N2 supplements (Gibco) at 37°C for 30 min. The digest was dissociated though a 1 ml pipette tip and passed through a 70 μM nylon mesh to obtain a single cell suspension. Cells were treated with Red Blood Cell Lysis buffer (Sigma) prior to further analysis.
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