Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV Document date: 2010_4_14
ID: 0xn6mqh6_11
Snippet: PCR efficiencies were calculated for each tissue culture cell line using a relative standard curve derived from a pooled cDNA mixture (a ten-fold dilution series with five measuring points). The pooled cDNA was obtained from control and infected samples from both CHSE-214 and RTS11 cell cultures, respectively, using the same RNA isolation and cDNA synthesis protocols as described above. The real-time PCR efficiencies were calculated from the slop.....
Document: PCR efficiencies were calculated for each tissue culture cell line using a relative standard curve derived from a pooled cDNA mixture (a ten-fold dilution series with five measuring points). The pooled cDNA was obtained from control and infected samples from both CHSE-214 and RTS11 cell cultures, respectively, using the same RNA isolation and cDNA synthesis protocols as described above. The real-time PCR efficiencies were calculated from the slope according to the established equation E = 10 (-1/slope) [4] .
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