Analysis of VSV pseudotype virus infection mediated by rubella virus envelope proteins

Sakata, Masafumi; Tani, Hideki; Anraku, Masaki; Kataoka, Michiyo; Nagata, Noriyo; Seki, Fumio; Tahara, Maino; Otsuki, Noriyuki; Okamoto, Kiyoko; Takeda, Makoto; Mori, Yoshio

In contrast, VSV GFP -RV/CE2E1 infectivity titers remained as low as those of VSV GFP -∆G in immune cell lines, and the infectivity titer, if any, of VSV GFP -RV/CE2E1 could not be detected in most immune cell lines (data not shown). To assess the susceptibility of the immune cell lines to VSV-RV/CE2E1 more sensitively, they were infected with VSV FLuc -RV/CE2E1, and any luciferase activity by the residual VSV FLuc -G was eliminated by the VSV G protein-specific antibody (Fig. 4D) . As was expected, the infectivity titers of VSV FLuc -RV/CE2E1 in immune cells were undetectable in many immune cell lines (Fig. 4D ). However, significant, but very low, levels of infectivity titers were detected in THP-1, Jurkat, and MT2 cells (Fig. 4D ).