Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway

Wang, Kai; Ran, Ling; Yan, Tao; Niu, Zheng; Kan, Zifei; Zhang, Yiling; Yang, Yang; Xie, Luyi; Huang, Shilei; Yu, Qiuhan; Wu, Di; Song, Zhenhui

IPEC-J2 cells were seeded at 1.8 × 10 6 /mL in 6-well plates, grown to 90% confluence, and then divided into three groups: Cells treated with Lp-1s for 1.5 h and then infected with TGEV (MOI = 0.1), TGEV infection alone, and the blank control (uninfected cells). The IPEC-J2 cells were sampled at 12, 24, and 48 hpi for qRT-PCR. Total RNA was extracted using the RNAiso plus reagent (Takara), and then single-stranded RNA was isolated using an RNA PCR (AMV) ver 3.0 kit (Takara). cDNA was then synthesized via reverse transcription. Quantitative real-time PCR was then performed using the SYBR premix EX Taq II (Takara) to detect the mRNA levels of ZAP, PKR, OASL, and ISG15 (fluorescent primers were synthesized by Shanghai Shenggong Bioengineering Technology Service Co., Ltd.).