Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Document date: 2017_9_21
ID: 1k9l0z5k_23
Snippet: To identify the conserved regions, the complete genome sequences of PDCoV currently available in GenBank, including KR131621, KX083667, KP757891, K981395, KJ567050, KT021234, KT266822, and KY065120, were analyzed by the Vector NTI ® Advance 10 program (Invitrogen Corp, USA). The conserved sequence fragment of the nucleocapsid (N) gene of PDCoV was selected as the targeted region, and used to design the PDCoV LAMP primers by the Primer Explorer V.....
Document: To identify the conserved regions, the complete genome sequences of PDCoV currently available in GenBank, including KR131621, KX083667, KP757891, K981395, KJ567050, KT021234, KT266822, and KY065120, were analyzed by the Vector NTI ® Advance 10 program (Invitrogen Corp, USA). The conserved sequence fragment of the nucleocapsid (N) gene of PDCoV was selected as the targeted region, and used to design the PDCoV LAMP primers by the Primer Explorer V4 software (http://primerexplorer.jp/e/). All the primers designed were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) with PAGE purification. The detailed information of the primers for the RT-LAMP is listed in Table 2 .
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