Author: Zhang, Fanfan; Ye, Yu; Song, Deping; Guo, Nannan; Peng, Qi; Li, Anqi; Zhou, Xingrong; Chen, Yanjun; Zhang, Min; Huang, Dongyan; Tang, Yuxin
Title: A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification Document date: 2017_9_21
ID: 1k9l0z5k_6
Snippet: The RT-LAMP assay for detection of PDCoV was successfully developed. The optimized parameters of the reaction system of the RT-LAMP were as follows: 6 mM MgSO 4 , 1.4 mM dNTPs, and 1.6 µM each of PDCoV-specific FIP and BIP primes with an incubation condition at 63 °C for 70 min in a water bath. The amplified products were electrophoresed on 2.0% agarose gel with addition of EB under UV light (Fig. 1a) . The RT-LAMP products were also visually d.....
Document: The RT-LAMP assay for detection of PDCoV was successfully developed. The optimized parameters of the reaction system of the RT-LAMP were as follows: 6 mM MgSO 4 , 1.4 mM dNTPs, and 1.6 µM each of PDCoV-specific FIP and BIP primes with an incubation condition at 63 °C for 70 min in a water bath. The amplified products were electrophoresed on 2.0% agarose gel with addition of EB under UV light (Fig. 1a) . The RT-LAMP products were also visually detected by adding SYBR Green I dye (Fig. 1b) .
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