Author: Griffiths, Samantha J.; Koegl, Manfred; Boutell, Chris; Zenner, Helen L.; Crump, Colin M.; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline C.; Barry, Gerald; Martin, Kim; Craigon, Marie H.; Chen, Rui; Kaza, Lakshmi N.; Fossum, Even; Fazakerley, John K.; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jürgen
Title: A Systematic Analysis of Host Factors Reveals a Med23-Interferon-? Regulatory Axis against Herpes Simplex Virus Type 1 Replication Document date: 2013_8_8
ID: 0lyt8gfq_60
Snippet: qRT-PCR analysis of interferon and cytokine induction A549 cells were transfected with 100 ng pCR3 or Med23 overexpression plasmids, or RSCF or Med23 SMARTpool siRNA (50 nM) in duplicates, in 96-well plates as described. RNA was harvested 20 h post-transfection, and mRNA expression levels quantified by qRT-PCR, as described......
Document: qRT-PCR analysis of interferon and cytokine induction A549 cells were transfected with 100 ng pCR3 or Med23 overexpression plasmids, or RSCF or Med23 SMARTpool siRNA (50 nM) in duplicates, in 96-well plates as described. RNA was harvested 20 h post-transfection, and mRNA expression levels quantified by qRT-PCR, as described.
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