Selected article for: "dual luciferase and firefly luciferase reporter"
Author: Griffiths, Samantha J.; Koegl, Manfred; Boutell, Chris; Zenner, Helen L.; Crump, Colin M.; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline C.; Barry, Gerald; Martin, Kim; Craigon, Marie H.; Chen, Rui; Kaza, Lakshmi N.; Fossum, Even; Fazakerley, John K.; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jürgen
Title: A Systematic Analysis of Host Factors Reveals a Med23-Interferon-? Regulatory Axis against Herpes Simplex Virus Type 1 Replication
  • Document date: 2013_8_8
    • ID: 0lyt8gfq_64
    Snippet: A point mutation (R611Q) was introduced into Med23 (Transcript Variant 1) by PCR with specific primers (see Table S9 in Text S2) and the clone verified by sequence analysis. A549 cells were co-transfected with 60 ng of pCR3, pCR3-Med23 or pCR3-R611Q, 60 ng of pCR3-IRF7, 20 ng of IFN-b-, IFN-l1-or ISRE-responsive luciferase reporter constructs and 10 ng pRL-TK, a Renilla Luciferase transfection control, in antibiotic-free low serum (1%) medium. A.....
    Document: A point mutation (R611Q) was introduced into Med23 (Transcript Variant 1) by PCR with specific primers (see Table S9 in Text S2) and the clone verified by sequence analysis. A549 cells were co-transfected with 60 ng of pCR3, pCR3-Med23 or pCR3-R611Q, 60 ng of pCR3-IRF7, 20 ng of IFN-b-, IFN-l1-or ISRE-responsive luciferase reporter constructs and 10 ng pRL-TK, a Renilla Luciferase transfection control, in antibiotic-free low serum (1%) medium. After 33 h cells were lysed, and firefly (promoter reporter construct) and Renilla (transfection control) luciferase activity was measured (Dual-Luciferase Reporter Kit, Promega). Relative luminescence activity was normalized to Renilla (as a transfection control).

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