Selected article for: "cell culture and clinical isolate"
Author: Claus, Claudia; Manssen, Lena; Hübner, Denise; Roßmark, Sarah; Bothe, Viktoria; Petzold, Alice; Große, Claudia; Reins, Mareen; Mankertz, Annette; Frey, Teryl K.; Liebert, Uwe G.
Title: Activation of the Mitochondrial Apoptotic Signaling Platform during Rubella Virus Infection
  • Document date: 2015_11_26
    • ID: 1rrm4sqa_36
    Snippet: Comparable to the vaccine strain HPV77, the selection of the Therien strain for its high replication rate in Vero cells was the result of a cell-culture adaptation process. CPE induction by a low-passaged clinical isolate, represented by the strain Wuerzburg-12 (Wb-12, RVi/Wuerzburg.DEU/47.11) was therefore addressed. The viral titer generated during Wb-12 infection is comparable to the one observed for Therien (representative graph in Figure 8A .....
    Document: Comparable to the vaccine strain HPV77, the selection of the Therien strain for its high replication rate in Vero cells was the result of a cell-culture adaptation process. CPE induction by a low-passaged clinical isolate, represented by the strain Wuerzburg-12 (Wb-12, RVi/Wuerzburg.DEU/47.11) was therefore addressed. The viral titer generated during Wb-12 infection is comparable to the one observed for Therien (representative graph in Figure 8A and [25] ). Additionally, CPE development by Wb-12 was comparable to Therien in terms of the onset of cell detachment. However, the number of floaters detected at 3 dpi per well of a six-well plate was slightly reduced for Wb-12 ((2.25¨2.29) ¢ 10 5 floaters per mL, n = 3) as compared to Therien (9.28¨2.25) ¢ 10 5 . Under Therien infection, only a slight shift of p53 to the nucleus was detectable ( Figure 5A ), which is comparable to the infection by HPV77, but more profound after infection with Wb-12 ( Figure 8B) .

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