Selected article for: "cDNA synthesis kit and synthesis kit"
Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens
  • Document date: 2016_8_9
    • ID: 16e99fuz_66
    Snippet: RNA from whole lung homogenates was isolated using a Trizol (Invitrogen) per manufacturer's protocol. cDNA was synthesized using the SuperScript VILO cDNA synthesis kit (Invitrogen) and the following parameters: 25°C for 10 min, 42°C for 1 hour, and 85°C for 5 min. PCR was run using 2 μl of diluted cDNA (1:5 in dH 2 O) to detect IFNa2/IFNa11 or IFNb mRNA using the Taqman primer/probe assay system (Life Technologies) per manufacturer's protoco.....
    Document: RNA from whole lung homogenates was isolated using a Trizol (Invitrogen) per manufacturer's protocol. cDNA was synthesized using the SuperScript VILO cDNA synthesis kit (Invitrogen) and the following parameters: 25°C for 10 min, 42°C for 1 hour, and 85°C for 5 min. PCR was run using 2 μl of diluted cDNA (1:5 in dH 2 O) to detect IFNa2/IFNa11 or IFNb mRNA using the Taqman primer/probe assay system (Life Technologies) per manufacturer's protocol. Samples were normalized to GAPDH mRNA expression as the internal control.

    Search related documents:
    Co phrase search for related documents
    • assay system and GAPDH mrna: 1
    • assay system and internal control: 1, 2, 3, 4, 5, 6, 7
    • assay system and Invitrogen cdna VILO synthesis kit: 1
    • assay system and Life Technologies assay system: 1, 2
    • assay system and primer probe: 1, 2
    • assay system and SuperScript Invitrogen cdna VILO synthesis kit: 1
    • assay system and synthesis kit: 1
    • cdna VILO synthesis kit and GAPDH mrna: 1
    • cdna VILO synthesis kit and internal control: 1
    • cdna VILO synthesis kit and Invitrogen cdna VILO synthesis kit: 1
    • cdna VILO synthesis kit and primer probe: 1
    • cdna VILO synthesis kit and SuperScript Invitrogen cdna VILO synthesis kit: 1
    • cdna VILO synthesis kit and synthesis kit: 1
    • GAPDH mrna and internal control: 1, 2, 3, 4, 5, 6
    • GAPDH mrna and Invitrogen cdna VILO synthesis kit: 1
    • GAPDH mrna and primer probe: 1, 2
    • GAPDH mrna and SuperScript Invitrogen cdna VILO synthesis kit: 1
    • GAPDH mrna and synthesis kit: 1, 2
    • GAPDH mrna and Taqman primer probe: 1