Selected article for: "cell replication and human infect"

Author: Lum, Fok-Moon; Couderc, Thérèse; Chia, Bing-Shao; Ong, Ruo-Yan; Her, Zhisheng; Chow, Angela; Leo, Yee-Sin; Kam, Yiu-Wing; Rénia, Laurent; Lecuit, Marc; Ng, Lisa F. P.
Title: Antibody-mediated enhancement aggravates chikungunya virus infection and disease severity
  • Document date: 2018_1_30
  • ID: 1vhzto1o_1
    Snippet: To investigate if sub-neutralizing concentrations of CHIKV-specific antibodies enhance CHIKV infection, diluted CHIKV-specific patients' plasma obtained from a CHIKV cohort 8, 34, 35 were mixed with CHIKV before being used to infect human primary monocytes and B cells. At low antibody concentration, antibody-mediated enhancement was shown to occur at antibody concentrations of 3.6 ± 2.9 μg/ml ( Table 1 ). The presence of CHIKV antigen was detec.....
    Document: To investigate if sub-neutralizing concentrations of CHIKV-specific antibodies enhance CHIKV infection, diluted CHIKV-specific patients' plasma obtained from a CHIKV cohort 8, 34, 35 were mixed with CHIKV before being used to infect human primary monocytes and B cells. At low antibody concentration, antibody-mediated enhancement was shown to occur at antibody concentrations of 3.6 ± 2.9 μg/ml ( Table 1 ). The presence of CHIKV antigen was detected by flow cytometry, where detection was increased by ~5 fold in monocytes (Fig. 1a ) and by ~20 fold in B cells (Fig. 1b) . However, active virus replication was not observed (Fig. 1c ,d) in both cell types. Next, a Zs-Green tagged CHIKV variant was used for the infection of human whole blood. With this virus, a successful infection would lead to the production of the Zs-Green protein. Levels of infection can therefore be known through the detection of Zs-Green positive cells. It was observed that infection in the presence of patients' plasma (total IgG concentrations of 1.8 ± 1.45 µg/ml) led to an increase in the numbers of Zs-Green positive monocytes. However, this was not observed in the B cells and plasmacytoid dendritic cells (pDCs) (Fig. S1a ). Once again, the viral RNA load did not concur with enhanced infection (Fig. S1b ).

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