Antibody Engineering for Pursuing a Healthier Future

Saeed, Abdullah F. U. H.; Wang, Rongzhi; Ling, Sumei; Wang, Shihua

Expression of recombinant antibodies in vitro experienced a boost with the advent of new molecular tools using various model organism such as yeast, bacteria etc., and new techniques for the selection of genetically engineered recombinant libraries using phage display technology. The phage display technique was first established by George P. Smith, when he validated the display of exogenous proteins on filamentous phage by fusing the peptide of interest to gene III of the phage. The first recombinant antibody fragments were constructed in bacteria 17 years ago (Roque et al., 2004) . The goal of antibody production technology is to achieve high-titers of highly specific, and highaffinity antisera. Antigen preparation and animal immunizations are carried out following the guidelines of production techniques via hybridoma technology and recombinant technology (Smith, 1985) . Moreover, therapeutic antibodies have been developed by modulation to the fragment crystallizable (Fc) receptor function and contribution of Fc glycan to immunoglobins, and the regulation of the antibody glycosylation in relation to immunoglobins-based therapeutics (Shade and Anthony, 2013) .