Author: Miguel, Maria Graça
Title: Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review Document date: 2010_12_15
ID: 1dfzj1b3_13
Snippet: There are two very popular methods for monitoring lipid peroxidation: determination of conjugated dienes (absorbance at 234 nm) and the TBARS (thiobarbituric acid reactive substances) assay. This method measures the malonaldehyde formed after lipid hydroperoxide decomposition, which forms a pink chromophore with thiobarbituric acid (TBA). However, TBARS is representative of a rather late stage of lipid oxidation, when the oxidation substrate has .....
Document: There are two very popular methods for monitoring lipid peroxidation: determination of conjugated dienes (absorbance at 234 nm) and the TBARS (thiobarbituric acid reactive substances) assay. This method measures the malonaldehyde formed after lipid hydroperoxide decomposition, which forms a pink chromophore with thiobarbituric acid (TBA). However, TBARS is representative of a rather late stage of lipid oxidation, when the oxidation substrate has undergone substantial transformation [29] . There are other methods, including the determination of peroxide value, iodine value, chromatographic measurement of volatile compounds, formic acid measurement [the Rancimat method in an automated test that measures the conductivity of low molecular weight fatty acids (formic acid) produced during the auto-oxidation of lipids at 100 ºC or above] [5] .
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