Author: Lum, Fok-Moon; Couderc, Thérèse; Chia, Bing-Shao; Ong, Ruo-Yan; Her, Zhisheng; Chow, Angela; Leo, Yee-Sin; Kam, Yiu-Wing; Rénia, Laurent; Lecuit, Marc; Ng, Lisa F. P.
Title: Antibody-mediated enhancement aggravates chikungunya virus infection and disease severity Document date: 2018_1_30
ID: 1vhzto1o_6
Snippet: have been hypothesized to act as a possible cellular vehicle and reservoir for virus dissemination and persistence [37] [38] [39] . Using human monocytes-derived macrophages (MDMs), which express high levels of FcγRI and II (Fig. 3a) , antibody-mediated enhancement of CHIKV infection was investigated with diluted patients' plasma (total IgG concentrations of 1.8 ± 1.45 µg/ml. In this experiment, infection was once again performed with the Zs-G.....
Document: have been hypothesized to act as a possible cellular vehicle and reservoir for virus dissemination and persistence [37] [38] [39] . Using human monocytes-derived macrophages (MDMs), which express high levels of FcγRI and II (Fig. 3a) , antibody-mediated enhancement of CHIKV infection was investigated with diluted patients' plasma (total IgG concentrations of 1.8 ± 1.45 µg/ml. In this experiment, infection was once again performed with the Zs-Green tagged CHIKV variant. It was observed that elevated levels of ZS-green positive cells were being detected at 24 hpi (Fig. 3b) . Likewise, it was also increased in primary monocytes isolated from the same donor (Fig. S2a) . Despite the increased level of Zs-green positive cells, viral RNA load remained unchanged ( Fig. 3c and S2b). Similarly, the enhancement observed was mediated via FcγRII, as blocking with FcγRII-specific antibodies completely abrogated infection (Fig. S3 ).
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