Author: Claus, Claudia; Manssen, Lena; Hübner, Denise; Roßmark, Sarah; Bothe, Viktoria; Petzold, Alice; Große, Claudia; Reins, Mareen; Mankertz, Annette; Frey, Teryl K.; Liebert, Uwe G.
Title: Activation of the Mitochondrial Apoptotic Signaling Platform during Rubella Virus Infection Document date: 2015_11_26
ID: 1rrm4sqa_5
Snippet: Floaters were collected at 3 dpi and then counted before the same number of floaters (1 ¢ 10 6 ) was subjected to DNA extraction and analysis by agarose gel electrophoresis. As a control, mock-and staurosporine (5 µM)-treated Vero cells are shown in comparison to the floater population (3 dpi) of RV-infected cells. M, molecular size marker; (D) Time course analysis (1, 2, 3 dpi) of the activation of caspase 3 and 7 in RV-infected Vero cells; (E.....
Document: Floaters were collected at 3 dpi and then counted before the same number of floaters (1 ¢ 10 6 ) was subjected to DNA extraction and analysis by agarose gel electrophoresis. As a control, mock-and staurosporine (5 µM)-treated Vero cells are shown in comparison to the floater population (3 dpi) of RV-infected cells. M, molecular size marker; (D) Time course analysis (1, 2, 3 dpi) of the activation of caspase 3 and 7 in RV-infected Vero cells; (E) Analysis of necrotic (Propidium iodide (PI)-positive) and early (annexin V-enhanced green fluorescent protein (EGFP)-positive) and late-apoptotic events (annexin V-enhanced green fluorescent protein (EGFP) and PI-positive) by annexin V-EGFP and PI staining by microscopic analysis of living cells; (F) Determination of caspase 3 and 7 activity after application of PFTµ and NIM811 in comparison to the SC. SC, solvent control; scale bar, 20 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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