Selected article for: "cell surface and MHC class"
Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens
  • Document date: 2016_8_9
    • ID: 16e99fuz_51
    Snippet: Staining. Equivalent cell numbers were treated with Fc block (BD Biosciences) and surface stained with combinations of anti-MHC II (M5/114.15.2), anti-CD11c (N418), anti-CD11b (M1/70), and anti-Ly6c (HK1.4) antibodies (eBiosciences) (neutrophils) or anti-Ly6g (1A8), anti-MHC class II (AF6-120.1), anti-CD11c (N418; eBioscience), anti-Ly6c (ER-MP20; AbD Serotech), anti-CD11b (M1/70; BD Biosciences), anti-CD4 (RM4.5; BD Biosciences), anti-NK1.1 (PK1.....
    Document: Staining. Equivalent cell numbers were treated with Fc block (BD Biosciences) and surface stained with combinations of anti-MHC II (M5/114.15.2), anti-CD11c (N418), anti-CD11b (M1/70), and anti-Ly6c (HK1.4) antibodies (eBiosciences) (neutrophils) or anti-Ly6g (1A8), anti-MHC class II (AF6-120.1), anti-CD11c (N418; eBioscience), anti-Ly6c (ER-MP20; AbD Serotech), anti-CD11b (M1/70; BD Biosciences), anti-CD4 (RM4.5; BD Biosciences), anti-NK1.1 (PK136; BD Biosciences), αβT cell receptor (H57-597; BD), anti-CD8 (53-6.7; eBioscience), anti-iNOS (M-19; Santa Cruz Biotechnology), or anti-TNF-α (MP6-XT22; BD Biosciences) (tipDCs, CD4 + T cells, CD8 + T cells) as previously described [37, 66] . CD8 + T cells were stained with tetramers specific for the D b PB1-F2 62-70 epitope for 1 h at room temperature prior to surface staining with the monoclonal antibodies as previously described [66] .

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