Selected article for: "relative gene expression and threshold cycle"
Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV
  • Document date: 2010_4_14
    • ID: 0xn6mqh6_13
    Snippet: The threshold cycle (Ct) values of the Opticon Monitor 2 software version 2.03 were transformed to relative quantities for analysis with the geNorm 3.5 software as described by Vandesompele et al. [3] . For the conversion of the Ct values to relative quantities [24] , reaction efficiencies were used. Relative gene expression for GAPDH, TUBA and EF1A were calculated using the geometric mean of the three most stable genes of each assay as normaliza.....
    Document: The threshold cycle (Ct) values of the Opticon Monitor 2 software version 2.03 were transformed to relative quantities for analysis with the geNorm 3.5 software as described by Vandesompele et al. [3] . For the conversion of the Ct values to relative quantities [24] , reaction efficiencies were used. Relative gene expression for GAPDH, TUBA and EF1A were calculated using the geometric mean of the three most stable genes of each assay as normalization factors and the 2d (in P. salmonis assays) and the 6 h (in IPNV assays) controls as calibrators, respectively.

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