Selected article for: "CT threshold cycle and threshold cycle"
Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV
  • Document date: 2010_4_14
    • ID: 0xn6mqh6_17
    Snippet: Prior validation of the selected reference gene candidates (ACTB, EF1A, GAPDH, UBQ and TUBA), general expression levels based on mean qPCR threshold cycle (Ct) values in control CHSE-214 and RTS11 cells were determined, since extremely high or low expression levels might preclude their usefulness as internal controls ( Figure 3) . Ct values from 16.17 and 24.99 indicated expression levels in an appropriate range......
    Document: Prior validation of the selected reference gene candidates (ACTB, EF1A, GAPDH, UBQ and TUBA), general expression levels based on mean qPCR threshold cycle (Ct) values in control CHSE-214 and RTS11 cells were determined, since extremely high or low expression levels might preclude their usefulness as internal controls ( Figure 3) . Ct values from 16.17 and 24.99 indicated expression levels in an appropriate range.

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