Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens Document date: 2016_8_9
ID: 16e99fuz_32
Snippet: Influenza virus. Four-to nine-week-old male and female C57BL/6 (WT) mice and β6 KO mice were lightly anesthetized by isoflurane and intranasally inoculated with PBS (control), CA/09 H1N1 (10 4 TCID 50 ), or H7N9 and H5N1 (10 2 TCID 50 ) influenza viruses. Mice were monitored at least daily for morbidity, weights recorded every 48 hours and tissues/BALF collected at different times post-infection. Mice were euthanized if they lost more than 30% o.....
Document: Influenza virus. Four-to nine-week-old male and female C57BL/6 (WT) mice and β6 KO mice were lightly anesthetized by isoflurane and intranasally inoculated with PBS (control), CA/09 H1N1 (10 4 TCID 50 ), or H7N9 and H5N1 (10 2 TCID 50 ) influenza viruses. Mice were monitored at least daily for morbidity, weights recorded every 48 hours and tissues/BALF collected at different times post-infection. Mice were euthanized if they lost more than 30% of starting weight and exhibited any symptoms of paralysis. H7N9 and H5N1 experiments were performed in a USDA-certified biosafety level 3 enhanced containment laboratory [63] .
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