Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens Document date: 2016_8_9
ID: 16e99fuz_36
Snippet: Fransicella tularensis challenge. F. tularensis SchuS4 was propagated as previously described [64] and grown in Mueller Hinton (MMH) broth overnight, aliquoted into 1 ml samples and frozen at -80°C. Immediately prior to use aliquots were thawed and serially diluted to the desired concentration in pharmaceutical grade saline. Inoculum doses were confirmed by plating diluted bacteria on MMH agar and enumerating colonies after 48 hours incubation a.....
Document: Fransicella tularensis challenge. F. tularensis SchuS4 was propagated as previously described [64] and grown in Mueller Hinton (MMH) broth overnight, aliquoted into 1 ml samples and frozen at -80°C. Immediately prior to use aliquots were thawed and serially diluted to the desired concentration in pharmaceutical grade saline. Inoculum doses were confirmed by plating diluted bacteria on MMH agar and enumerating colonies after 48 hours incubation at 37°C. Mice were anesthetized by a single intraperitoneal injection of 12.5 mg/ml ketamine + 3.8 mg/ml xylazine and immediately inoculated intranasally with 25 CFU F. tularensis SchuS4. All mice were monitored daily for signs of illness.
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