Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_22
Snippet: LiCl and DG inhibit PDCoV replication. Next, we investigated the effect of LiCl and DG on the replication of PDCoV in LLC-PK1 cells. PK-1 cells were infected with PDCoV at MOI 0.05 for 1 h and treated with 10-60 mM LiCl or 125-1250 µg/mL DG for 24 h. The relative mRNA expression was detected by real-time qPCR. Treatment with 10-60 mM LiCl or 125-1250 µg/mL DG inhibited viral mRNA levels significantly (Figures 2A and 3A) . Infectious virus loads.....
Document: LiCl and DG inhibit PDCoV replication. Next, we investigated the effect of LiCl and DG on the replication of PDCoV in LLC-PK1 cells. PK-1 cells were infected with PDCoV at MOI 0.05 for 1 h and treated with 10-60 mM LiCl or 125-1250 µg/mL DG for 24 h. The relative mRNA expression was detected by real-time qPCR. Treatment with 10-60 mM LiCl or 125-1250 µg/mL DG inhibited viral mRNA levels significantly (Figures 2A and 3A) . Infectious virus loads in cell culture supernatants were determined by TCID 50 . We found that 10-60 mM LiCl or 125-1250 µg/mL DG significantly inhibited viral replication compared to mock-treated cells ( Figures 2B and 3B ). In IFA, the fluorescence intensity declined after treatment with 10-60 mM LiCl or 125-1250 µg/mL DG in a dose-dependent manner ( Figures 2C and 3C ). The fluorescence intensity was quantified, showing that the number of infected cells decreased in a drug dose-dependent manner ( Figures 2D and 3D ). These results indicate that LiCl and DG inhibit PDCoV replication in a dose-dependent manner.
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