Author: Perdomo, German; Dong, H. Henry
Title: Apolipoprotein D in Lipid Metabolism and Its Functional Implication in Atherosclerosis and Aging Document date: 2008_12_12
ID: 167z915s_38
Snippet: Analysis of apoD N-glycosylation. Aliquots of plasma (20 µg protein) from C57BL/6J mice (male, 10 weeks old) were incubated without (-) and with (+) 1,000 U of N-glycosidase F (New England Biolabs) in a total volume of 30 µl at 37°C for 1 hour. The reaction mixture was resolved on 4-20% SDS-polyacrylamide gels, followed by immunoblot analysis using polyclonal rabbit anti-apoD (developed in our own laboratory)......
Document: Analysis of apoD N-glycosylation. Aliquots of plasma (20 µg protein) from C57BL/6J mice (male, 10 weeks old) were incubated without (-) and with (+) 1,000 U of N-glycosidase F (New England Biolabs) in a total volume of 30 µl at 37°C for 1 hour. The reaction mixture was resolved on 4-20% SDS-polyacrylamide gels, followed by immunoblot analysis using polyclonal rabbit anti-apoD (developed in our own laboratory).
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