Selected article for: "bovine serum albumin and PBS bovine serum albumin"
Author: Shenglan Shang; Jiaqi Wu; Xiaoli Li; Xin Liu; Pan Li; Chunli Zheng; Yonghua Wang; Songqing Liu; Jiang Zheng; Hong Zhou
Title: Artesunate interacts with Vitamin D receptor to reverse mouse model of sepsis-induced immunosuppression via enhancing autophagy
  • Document date: 2020_2_27
    • ID: egntml7e_32
    Snippet: RAW264.7 cells or PMs plated on the coverslips for 2 h and treated as indicated in Method 2.5. The coverslips were fixed in 4% paraformaldehyde for 20 min, and permeabilized in 0.5% Triton X-100 (Solarbio, Beijing, China) which dissolved in PBS for 5 min (20min for nucleoprotein staining).Then the coverslips were blocked for 1 h at 37°C in PBS containing 3% bovine serum albumin (BSA) and 0.1% Triton X-100, and incubated with primary antibodies (.....
    Document: RAW264.7 cells or PMs plated on the coverslips for 2 h and treated as indicated in Method 2.5. The coverslips were fixed in 4% paraformaldehyde for 20 min, and permeabilized in 0.5% Triton X-100 (Solarbio, Beijing, China) which dissolved in PBS for 5 min (20min for nucleoprotein staining).Then the coverslips were blocked for 1 h at 37°C in PBS containing 3% bovine serum albumin (BSA) and 0.1% Triton X-100, and incubated with primary antibodies (displayed in Supplementary Table 1) at 4 °C overnight, followed by secondary antibodies.

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