Author: Jones, Harrison G.; Battles, Michael B.; Lin, Chun-Chi; Bianchi, Siro; Corti, Davide; McLellan, Jason S.
Title: Alternative conformations of a major antigenic site on RSV F Document date: 2019_7_15
ID: 1r20hl2b_39
Snippet: A molecular replacement solution for the 3.50 Ã… complex of DS-Cav1 with AM22 Fab was obtained using PHASER with prefusion-stabilized RSV F variant Cav1 (PDB ID: 4MMS) and the 1.70 Ã… AM22 Fab structures as search models. The asymmetric unit contained the prefusion trimer bound by three AM22 Fabs. Rigid-body refinement was then performed in PHE-NIX, followed by refining group B-factors and (x, y, z) coordinates in PHENIX with NCS torsion restrain.....
Document: A molecular replacement solution for the 3.50 Ã… complex of DS-Cav1 with AM22 Fab was obtained using PHASER with prefusion-stabilized RSV F variant Cav1 (PDB ID: 4MMS) and the 1.70 Ã… AM22 Fab structures as search models. The asymmetric unit contained the prefusion trimer bound by three AM22 Fabs. Rigid-body refinement was then performed in PHE-NIX, followed by refining group B-factors and (x, y, z) coordinates in PHENIX with NCS torsion restraints and reference-model restraints. The reference model was the 2.40 Ã… prefusion-stabilized RSV F variant Cav1 (PDB ID: 4MMS). The structure was built and refined to an R work /R free of 21.7%/28.0% (Table 1) .
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