Selected article for: "blue dye and endothelial permeability"
Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens
  • Document date: 2016_8_9
    • ID: 16e99fuz_47
    Snippet: Endothelial permeability assay. At 8 dpi, mice were administered 20 mg/kg Evans blue dye (Sigma) by retro-orbital injection. The dye was allowed to circulate for 2 h before mice were sacrificed and perfused with 10 ml PBS. Lungs were harvested and incubated in formamide (Sigma) at 37°C. After 48 h, lungs were removed from formamide and dried at 65°C for an additional 96 h before being weighed. Evans blue dye absorbance was measured by spectroph.....
    Document: Endothelial permeability assay. At 8 dpi, mice were administered 20 mg/kg Evans blue dye (Sigma) by retro-orbital injection. The dye was allowed to circulate for 2 h before mice were sacrificed and perfused with 10 ml PBS. Lungs were harvested and incubated in formamide (Sigma) at 37°C. After 48 h, lungs were removed from formamide and dried at 65°C for an additional 96 h before being weighed. Evans blue dye absorbance was measured by spectrophotometry at 620 nm. The lung permeability index [65] was calculated using the following formula: [absorbance / dry lung weight (g)].

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