Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV Document date: 2010_4_14
ID: 0xn6mqh6_6
Snippet: Experiments with IPNV were performed using the Chilean strain VR299. The virus was propagated by inoculating CHSE-214 cell monolayers at a multiplicity of infection (MOI) of 0.1 to 1 PFU/cell in MEM supplemented with 2% FBS and antibiotics. Infected cultures were incubated at 17°C and monitored until CPE was evident and the clarified supernatants were divided into aliquots that were stored at -20°C. Aliquots were titrated in a plaque formation .....
Document: Experiments with IPNV were performed using the Chilean strain VR299. The virus was propagated by inoculating CHSE-214 cell monolayers at a multiplicity of infection (MOI) of 0.1 to 1 PFU/cell in MEM supplemented with 2% FBS and antibiotics. Infected cultures were incubated at 17°C and monitored until CPE was evident and the clarified supernatants were divided into aliquots that were stored at -20°C. Aliquots were titrated in a plaque formation assay as described previously [21] . For expression studies, both CHSE-214 and RTS11 cultures were inoculated at a MOI of 1×, and cells were harvested at 6 h, 24 h and 48 h post-infection.
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