Author: Labrie, Marilyne; Lalonde, Simon; Najyb, Ouafa; Thiery, Maxime; Daneault, Caroline; Des Rosiers, Chrisitne; Rassart, Eric; Mounier, Catherine
Title: Apolipoprotein D Transgenic Mice Develop Hepatic Steatosis through Activation of PPAR? and Fatty Acid Uptake Document date: 2015_6_17
ID: 0wtq1c15_16
Snippet: To measure the concentration of Prostaglandin E2 (PGE2), whole blood samples were collected from 1 year-old WT and Tg mice by cardiac puncture using heparinized syringe and kept on ice. Plasma was isolated by centrifugation (2000 RPM for 10 minutes at 4°C) and stored at -80°C. Liver extracts were prepared by homogenizing tissues in cold lysis buffer (50 mM Tris-HCl pH 7.3, 150 mM NaCl, 5 mM EDTA, 0.2% Triton X-100, 2 mM sodium orthovanadate and.....
Document: To measure the concentration of Prostaglandin E2 (PGE2), whole blood samples were collected from 1 year-old WT and Tg mice by cardiac puncture using heparinized syringe and kept on ice. Plasma was isolated by centrifugation (2000 RPM for 10 minutes at 4°C) and stored at -80°C. Liver extracts were prepared by homogenizing tissues in cold lysis buffer (50 mM Tris-HCl pH 7.3, 150 mM NaCl, 5 mM EDTA, 0.2% Triton X-100, 2 mM sodium orthovanadate and 10% Complete protease inhibitor). Lysates were then incubated 30 min at 4°C, cleared by centrifugation and stored at -80°C. The concentration of PGE2 was then measured using a specific immunoassay (Enzo Life Sciences, ADI-900-001) according to the manufacturer protocol.
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