Author: Labrie, Marilyne; Lalonde, Simon; Najyb, Ouafa; Thiery, Maxime; Daneault, Caroline; Des Rosiers, Chrisitne; Rassart, Eric; Mounier, Catherine
Title: Apolipoprotein D Transgenic Mice Develop Hepatic Steatosis through Activation of PPAR? and Fatty Acid Uptake Document date: 2015_6_17
ID: 0wtq1c15_9
Snippet: The human hepatocarcinoma cells (HepG2) were cultured in Eagle's Minimum Essential Medium (EMEM) supplemented with 10% FBS. Cells were then transfected with a UAS-Luciferase construct (a luciferase reporter plasmid containing five PPAR response elements) in combination with a human myc-tag apoD cDNA construct [43] (or an empty vector) in the presence or not of Gal4-PPARγ (containing the PPARγ cDNA and the DNA binding domain of GAL4) using Fugen.....
Document: The human hepatocarcinoma cells (HepG2) were cultured in Eagle's Minimum Essential Medium (EMEM) supplemented with 10% FBS. Cells were then transfected with a UAS-Luciferase construct (a luciferase reporter plasmid containing five PPAR response elements) in combination with a human myc-tag apoD cDNA construct [43] (or an empty vector) in the presence or not of Gal4-PPARγ (containing the PPARγ cDNA and the DNA binding domain of GAL4) using Fugene HD transfection reagent. 48h after the transfection, cells were incubated for 4h with 7μM of AA bound to BSA at a mole ratio AA:BSA 4:1. Cells were then harvested and cellular extracts were prepared for luciferase [45] and β-galactosidase assays (Invitrogen).
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