Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_1
Snippet: The PDCoV strain SD2018/10 from our lab was used in this study. The number of infectious PDCoV particles was determined on the basis of the 50% tissue culture infectious dose (TCID 50 ) in LLC-PK1 cells. Briefly, the cells were seeded into 96-cell plates and grown to 100% confluence for 24 h. Then, the cells were infected using 10-fold serial dilutions of virus samples. After incubation at 37 • C for 2 h, the cells were washed three times with .....
Document: The PDCoV strain SD2018/10 from our lab was used in this study. The number of infectious PDCoV particles was determined on the basis of the 50% tissue culture infectious dose (TCID 50 ) in LLC-PK1 cells. Briefly, the cells were seeded into 96-cell plates and grown to 100% confluence for 24 h. Then, the cells were infected using 10-fold serial dilutions of virus samples. After incubation at 37 • C for 2 h, the cells were washed three times with PBS and incubated for 72 h. Titers are reported as TCID 50 calculated by the method of Reed and Muench.
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