Author: Perdomo, German; Dong, H. Henry
Title: Apolipoprotein D in Lipid Metabolism and Its Functional Implication in Atherosclerosis and Aging Document date: 2008_12_12
ID: 167z915s_39
Snippet: RNA isolation and RT-PCR assay. Total RNA isolation from tissue (20 mg) was performed using the RNeasy Mini Kit (QIAGEN, Valencia, CA). Aliquots of purified RNA (100 ng) from were subjected to RT-PCR analysis using apoD sequence-specific primers flanking the apoD mRNA for forward reaction (5'-TAAGGCCTCTCCTGCAGCCA-3') and reverse reaction (5'-CTTTACAGGAAGTCCGGGCAG-3'). The resulting PCR products were resolved on 1% agarose gel containing ethidium .....
Document: RNA isolation and RT-PCR assay. Total RNA isolation from tissue (20 mg) was performed using the RNeasy Mini Kit (QIAGEN, Valencia, CA). Aliquots of purified RNA (100 ng) from were subjected to RT-PCR analysis using apoD sequence-specific primers flanking the apoD mRNA for forward reaction (5'-TAAGGCCTCTCCTGCAGCCA-3') and reverse reaction (5'-CTTTACAGGAAGTCCGGGCAG-3'). The resulting PCR products were resolved on 1% agarose gel containing ethidium bromide and visualized by UV light.
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