Selected article for: "agarose gel and Qiagen Valencia mini kit"
Author: Perdomo, German; Dong, H. Henry
Title: Apolipoprotein D in Lipid Metabolism and Its Functional Implication in Atherosclerosis and Aging
  • Document date: 2008_12_12
    • ID: 167z915s_39
    Snippet: RNA isolation and RT-PCR assay. Total RNA isolation from tissue (20 mg) was performed using the RNeasy Mini Kit (QIAGEN, Valencia, CA). Aliquots of purified RNA (100 ng) from were subjected to RT-PCR analysis using apoD sequence-specific primers flanking the apoD mRNA for forward reaction (5'-TAAGGCCTCTCCTGCAGCCA-3') and reverse reaction (5'-CTTTACAGGAAGTCCGGGCAG-3'). The resulting PCR products were resolved on 1% agarose gel containing ethidium .....
    Document: RNA isolation and RT-PCR assay. Total RNA isolation from tissue (20 mg) was performed using the RNeasy Mini Kit (QIAGEN, Valencia, CA). Aliquots of purified RNA (100 ng) from were subjected to RT-PCR analysis using apoD sequence-specific primers flanking the apoD mRNA for forward reaction (5'-TAAGGCCTCTCCTGCAGCCA-3') and reverse reaction (5'-CTTTACAGGAAGTCCGGGCAG-3'). The resulting PCR products were resolved on 1% agarose gel containing ethidium bromide and visualized by UV light.

    Search related documents:
    Co phrase search for related documents
    • agarose gel and ethidium bromide: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • agarose gel and forward reaction: 1
    • agarose gel and Mini kit: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • agarose gel and PCR product: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • agarose gel and reaction reverse: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • agarose gel and RNA isolation: 1, 2, 3, 4, 5, 6
    • agarose gel and RNeasy Mini kit: 1, 2, 3
    • agarose gel and RT PCR analysis: 1, 2, 3, 4, 5, 6
    • agarose gel and RT PCR assay: 1, 2, 3, 4, 5
    • agarose gel and uv light: 1, 2, 3, 4, 5, 6, 7
    • agarose gel and Valencia QIAGEN RNeasy Mini kit: 1
    • apod sequence and ethidium bromide: 1
    • apod sequence and RT PCR analysis: 1
    • apod sequence and uv light: 1
    • ethidium bromide and Mini kit: 1
    • ethidium bromide and PCR product: 1, 2, 3, 4
    • ethidium bromide and reaction reverse: 1, 2, 3, 4
    • ethidium bromide and RNeasy Mini kit: 1
    • ethidium bromide and RT PCR analysis: 1