Author: Peña, Andrea A; Bols, Niels C; Marshall, Sergio H
Title: An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV Document date: 2010_4_14
ID: 0xn6mqh6_12
Snippet: Real-time PCR were assayed on every biological replicate and each sample was run in duplicate. Each PCR reaction included reverse transcriptase negative controls for testing genomic DNA contamination and a non template negative control to check for primer dimer. To minimize experimental variation, each gene was quantified on the same batch of cDNA and the same gene was tested on the different samples in the same PCR run......
Document: Real-time PCR were assayed on every biological replicate and each sample was run in duplicate. Each PCR reaction included reverse transcriptase negative controls for testing genomic DNA contamination and a non template negative control to check for primer dimer. To minimize experimental variation, each gene was quantified on the same batch of cDNA and the same gene was tested on the different samples in the same PCR run.
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