Author: Wang, Kai; Ran, Ling; Yan, Tao; Niu, Zheng; Kan, Zifei; Zhang, Yiling; Yang, Yang; Xie, Luyi; Huang, Shilei; Yu, Qiuhan; Wu, Di; Song, Zhenhui
Title: Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway Document date: 2019_11_6
ID: 05tf6oqa_25
Snippet: Small interfering RNA (siRNA) targeting STAT1, 5 -GGAACAGAAATACACCTAT-3 (produced by RIBO, China) . Transfected with the STAT1-specific siRNA using LipofectamineTM 3000 (Invitrogen, United States), according to the manufacturer's instructions, to the TGEV-infected groups treated with Lp-1s and TGEV infection alone groups, respectively, in IPEC-J2 cells. ConsiRNA transfected as control groups. The collected samples were analyzed by Western blottin.....
Document: Small interfering RNA (siRNA) targeting STAT1, 5 -GGAACAGAAATACACCTAT-3 (produced by RIBO, China) . Transfected with the STAT1-specific siRNA using LipofectamineTM 3000 (Invitrogen, United States), according to the manufacturer's instructions, to the TGEV-infected groups treated with Lp-1s and TGEV infection alone groups, respectively, in IPEC-J2 cells. ConsiRNA transfected as control groups. The collected samples were analyzed by Western blotting using a rabbit pAb recognizing STAT1 as the primary antibody and HRP-conjugated goat anti-rabbit IgG as the secondary antibody.
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