Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway

Wang, Kai; Ran, Ling; Yan, Tao; Niu, Zheng; Kan, Zifei; Zhang, Yiling; Yang, Yang; Xie, Luyi; Huang, Shilei; Yu, Qiuhan; Wu, Di; Song, Zhenhui

The GenBank sequences of the TGEV N gene (GQ-374566.1) coding sequence (CDS) conserved region, the porcine MX1 CDS (MX dynamin like GTPase 1; AH015318.2), the MX2 CDS (MX dynamin like GTPase 2; AY897395.1), the ISG15 CDS (interferon-stimulated protein, 15 KDa; NM_214303.2), the OASL CDS (2 -5 -oligoadenylate synthetase like; NM_214303.2), the PKR CDS (double stranded RNA-dependent protein kinase; AB104654.1), the ZAP CDS (zeta-chain associated protein; GU_563332.1), and internal reference pig β-Actin gene (ACTB; XM_003124280.2) were obtained and pairs of specific primers were designed using Primer 5.0 software for quantitative realtime PCR [performed using SYBR Premix EX Taq II (Takara, Shiga, Japan)] as follows: TGEV-N (Forward: 5 -TTCAACCCC ATAACCCTCCAACAA-3 and Reverse: 5 -GGCCCTTCAC CAT GCGATAGC-3 ), MX1 (Forward: 5 -ATCTGTAAGCAGG AGACCATCAACTT G-3 and Reverse: 5 -CTCGCCACGTCCA CTATCTTGTC-3 ), MX2 (Forward: 5 -TTCACTCGCATCCGC ACTTCAG-3 and Reverse: 5 -AGCTCCTCTGTCGCACTC TGG-3 ), ISG15 (Forward: 5 -GGCAGCACAGTCCTGTT GATGG-3 and Reverse: 5 -TGCGTCAGCCAGACCTCAT AGG-3 ), OASL (Forward: 5 -CGTTGGTGGTGG AGACACA TACAG-3 and Reverse: 5 -TCAGGCGACACCTTCCAGG ATC-3 ), PKR (Forward: 5 -ACAGGACCTGCACATAACT TGAGG-3 and Reverse: 5 -TGCTGTCGGCAGTGATGAAGA AC-3 ), ZAP (Forward: 5 -GCTCAGTGCGAAC ACCTGGA TG-3 and Reverse: 5 -TGACAGATGAAGGCGTGGAG AGG-3 ), and ACTB (Forward: 5 -CTCTTCCAGC CCTCCT TCC-3 and Reverse: 5 -GGTCCTTG CGGATGTCG-3 ). The designed primers were synthesized by Shanghai Shenggong Biotechnology Service Co., Ltd. (Shanghai, China).