Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway

Wang, Kai; Ran, Ling; Yan, Tao; Niu, Zheng; Kan, Zifei; Zhang, Yiling; Yang, Yang; Xie, Luyi; Huang, Shilei; Yu, Qiuhan; Wu, Di; Song, Zhenhui

The results in Figure 6 show that the amount of p-STAT1 (red fluorescence) in the nuclei of TGEV infected cells treated with Lp-1s at different time points was significantly higher than that in the nuclei of cells in TGEV infected group. At the same time, the amount of p-STAT1 correlated positively with the duration Frontiers in Microbiology | www.frontiersin.org FIGURE 1 | MTT cytotoxicity test and Lp-1s concentration screening. (A) Results of Lp-1s cytotoxicity as detected using the MTT method on 1/4, 1/8, 1/32, and 1/64 times dilutions of Lp-1s acting on IPEC-J2 cells. The cell adherence state remained basically unchanged. According to the experimental data, the Lp-1s undiluted group, the 1/2-fold dilution group and the 1/4-fold dilution group showed significantly difference in cytotoxicity ( * * P < 0.01), and the 1/4-fold dilution group showed no significant difference compared with that of the control group (P > 0.05). (B) The expression of the TGEV N protein in IPEC-J2 cells treated with Lp-1s at different dilutions was detected by western blotting. Lane 1, infected TGEV group after Lp-1s 1/4 dilution pretreatment; Lane 2, infected TGEV group after Lp-1s 1/8 dilution pretreatment; Lane 3, infected TGEV group after Lp-1s 1/16 dilution pretreatment; Lane 4, infected TGEV group after Lp-1s 1/32 dilution pretreatment; Lane 5, TGEV infection group; and Lane 6, uninfected control cells (normal group). (C) Grayscale analysis of the relative expression of TGEV N in IPEC-J2 cells infected with Lp-1s at different dilutions showing that TGEV N protein levels were decreased in the 1/4-fold dilution of Lp-1s treatment group after 48 h, compared with the 1/8-fold dilution and TGEV infection group, there was a significant difference ( * * P < 0.01), which was significantly different from the control group ( * P < 0.05).