Selected article for: "fetal bovine serum and viral replication"

Author: Wang, Kai; Ran, Ling; Yan, Tao; Niu, Zheng; Kan, Zifei; Zhang, Yiling; Yang, Yang; Xie, Luyi; Huang, Shilei; Yu, Qiuhan; Wu, Di; Song, Zhenhui
Title: Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway
  • Document date: 2019_11_6
  • ID: 05tf6oqa_5
    Snippet: Porcine kidney cells (ST) to amplify the virus and the experimental model pig jejunal cells (IPEC-J2 cells) were both cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, Grand Island, NY, United States) containing 10% fetal bovine serum (FBS, Gibco), under 37 • C and 5% CO 2 . IPEC-J2 cells and ST cells were purchased from the Shanghai Sur Biotech Co., Ltd (Shanghai, China). L. plantarum LP-1 was isolated and stored in our la.....
    Document: Porcine kidney cells (ST) to amplify the virus and the experimental model pig jejunal cells (IPEC-J2 cells) were both cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, Grand Island, NY, United States) containing 10% fetal bovine serum (FBS, Gibco), under 37 • C and 5% CO 2 . IPEC-J2 cells and ST cells were purchased from the Shanghai Sur Biotech Co., Ltd (Shanghai, China). L. plantarum LP-1 was isolated and stored in our laboratory. Its 16S ribosomal gene sequence has been submitted to GenBank (MH727586). Lp-1s was isolated from a culture of L. plantarum LP-1 after shaking for 14 h at 37 • C. The TGEV Miller strain was preserved in our laboratory. Viral fluid was collected from ST cells after replication for approximately 72 h when the cells showed obvious cytopathic effects (CPEs).

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