Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology Document date: 2011_11_14
ID: 01nuj0lk_14
Snippet: A comparative study of the analytical sensitivity of the two platforms, 454 pyrosequencing and Illumina GA, for the detection of viruses in biological samples was done on a set of samples which were artificially spiked with eleven different viruses [35] . The Illumina method had a much greater sensitivity than 454, approaching that of optimized quantitative real-time PCR. However, at low viral concentration in the specimen, the number of reads ge.....
Document: A comparative study of the analytical sensitivity of the two platforms, 454 pyrosequencing and Illumina GA, for the detection of viruses in biological samples was done on a set of samples which were artificially spiked with eleven different viruses [35] . The Illumina method had a much greater sensitivity than 454, approaching that of optimized quantitative real-time PCR. However, at low viral concentration in the specimen, the number of reads generated by the Illumina platform was too small for de novo assembly of viral genome sequences [35] .
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