Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology Document date: 2011_11_14
ID: 01nuj0lk_21
Snippet: The Papillomaviridae family includes several viral species and at least 189 completely characterized papillomavirus types and putative new types are continuously found [45] . High throughput 454 pyrosequencing of amplicons generated by consensus PCR of a conserved region of viral genome was used to detect and genotype HPV in cervical cytology specimens [46] . The method allowed the detection of HPV types which were present in low amount in multip.....
Document: The Papillomaviridae family includes several viral species and at least 189 completely characterized papillomavirus types and putative new types are continuously found [45] . High throughput 454 pyrosequencing of amplicons generated by consensus PCR of a conserved region of viral genome was used to detect and genotype HPV in cervical cytology specimens [46] . The method allowed the detection of HPV types which were present in low amount in multiple infections and had the potentiality to detect a broad spectrum of HPV types, subtypes, and variants [46] . A similar approach was used to detect and genotype cutaneous HPV types in a large series of squamous cell carcinoma of the skin and other skin lesions [47] . Several different HPV types were detected, including novel putative cutaneous HPVs [47] .
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