Selected article for: "drug resistance and treatment failure"
Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology
  • Document date: 2011_11_14
    • ID: 01nuj0lk_41
    Snippet: Conventional direct sequencing of RT-PCR products (referred to as "population sequencing") is the gold standard in HIV resistance testing and is used to detect drug-resistance mutations in the molecular targets of HIV-1 therapy, i.e., reverse transcriptase, protease, integrase, and V3 loop of the HIV env gene. A major limitation of direct PCR sequencing, however, is its inability to detect drug-resistant variants present in less than 20-25% of th.....
    Document: Conventional direct sequencing of RT-PCR products (referred to as "population sequencing") is the gold standard in HIV resistance testing and is used to detect drug-resistance mutations in the molecular targets of HIV-1 therapy, i.e., reverse transcriptase, protease, integrase, and V3 loop of the HIV env gene. A major limitation of direct PCR sequencing, however, is its inability to detect drug-resistant variants present in less than 20-25% of the heterogeneous virus population existing in a patient's plasma sample [96] . Several studies have shown that minor drug-resistant variants that are not detected by population-based sequencing are clinically relevant in that they are often responsible for the virological failure of a new antiretroviral treatment regimen [97] [98] [99] .

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