Author: Gwon, Yong-Dae; Strand, Mårten; Lindqvist, Richard; Nilsson, Emma; Saleeb, Michael; Elofsson, Mikael; Överby, Anna K.; Evander, Magnus
Title: Antiviral Activity of Benzavir-2 against Emerging Flaviviruses Document date: 2020_3_22
ID: 0ym40eki_24
Snippet: To generate the ZIKV NS5 gene standard curve, a 105 bp ZIKV NS5 PCR amplicon was cloned into a pJET1.2 vector (Thermo Fisher, Waltham, MA, USA). Logarithms (base 10) of concentrations of copy numbers were prepared from 10 9 to 10 1 . The qPCR reaction was performed and obtained Ct values were converted to a standard curve by using GraphPad Prism 7.0 software (GraphPad Software, San Diego, CA, USA). The detection limit was 1000 copies of the ZIKV .....
Document: To generate the ZIKV NS5 gene standard curve, a 105 bp ZIKV NS5 PCR amplicon was cloned into a pJET1.2 vector (Thermo Fisher, Waltham, MA, USA). Logarithms (base 10) of concentrations of copy numbers were prepared from 10 9 to 10 1 . The qPCR reaction was performed and obtained Ct values were converted to a standard curve by using GraphPad Prism 7.0 software (GraphPad Software, San Diego, CA, USA). The detection limit was 1000 copies of the ZIKV NS5 gene.
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