Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_11
Snippet: The cells were infected with PDCoV (MOI 0.05) at 4 • C for 1 h. Unbound viruses were removed by washing with cold PBS. The cells were then incubated with LiCl (60 mM) or DG (1250 µg/mL) at 37 • C for 2 h. As a control, another set of cells were infected with the same dose of PDCoV without drug treatment. After removing the unbound viruses by washing with cold PBS (pH 3.0), the levels of vRNA in cells were determined by real-time qPCR......
Document: The cells were infected with PDCoV (MOI 0.05) at 4 • C for 1 h. Unbound viruses were removed by washing with cold PBS. The cells were then incubated with LiCl (60 mM) or DG (1250 µg/mL) at 37 • C for 2 h. As a control, another set of cells were infected with the same dose of PDCoV without drug treatment. After removing the unbound viruses by washing with cold PBS (pH 3.0), the levels of vRNA in cells were determined by real-time qPCR.
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