Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro Document date: 2019_9_9
ID: 1m5yr8uc_25_1
Snippet: riment, drugs and virus were added together to LLC-PK1 cells for 1 h. The cells were then washed, and the incubation was continued in the presence of the drugs. In the other experiments, LLC-PK1 cells were infected with PDCoV for 1 h, the cells were then washed, and the drugs were added at 1, 3, 6, 9, 12, 16, and 20 hpi. In all experiments, the cells were infected with PDCoV [ multiplicity of infection (MOI) = 0.05], and 60 mM LiCl or 1250 μg/mL.....
Document: riment, drugs and virus were added together to LLC-PK1 cells for 1 h. The cells were then washed, and the incubation was continued in the presence of the drugs. In the other experiments, LLC-PK1 cells were infected with PDCoV for 1 h, the cells were then washed, and the drugs were added at 1, 3, 6, 9, 12, 16, and 20 hpi. In all experiments, the cells were infected with PDCoV [ multiplicity of infection (MOI) = 0.05], and 60 mM LiCl or 1250 μg/mL DG was added. The viral contents were significantly reduced in cells treated with LiCl at 0 to 16 h, but no significant reduction was detected after 16 h ( Figure 6B ). For DG, the latest effective time point of drug treatment was 12 hpi ( Figure 6C ). Therefore, we can conclude that the antiviral effects of LiCl and DG occur at the early stages of PDCoV replication.
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