Selected article for: "drug resistance and NGS method"
Author: Barzon, Luisa; Lavezzo, Enrico; Militello, Valentina; Toppo, Stefano; Palù, Giorgio
Title: Applications of Next-Generation Sequencing Technologies to Diagnostic Virology
  • Document date: 2011_11_14
    • ID: 01nuj0lk_45
    Snippet: Deep sequencing based on the 454 technology has been also applied for the detection of nucleoside and nucleotide reverse-transcriptase inhibitor resistance in HBV. The NGS method was more sensitive for the detection of rare HBV drug resistance mutations than conventional methods based on population sequencing or reverse hybridization [124, 125] . In addition, deep sequencing allowed to identify G-to-A hypermutation mediated by the apolipoprotein .....
    Document: Deep sequencing based on the 454 technology has been also applied for the detection of nucleoside and nucleotide reverse-transcriptase inhibitor resistance in HBV. The NGS method was more sensitive for the detection of rare HBV drug resistance mutations than conventional methods based on population sequencing or reverse hybridization [124, 125] . In addition, deep sequencing allowed to identify G-to-A hypermutation mediated by the apolipoprotein B mRNA editing enzyme, which was estimated to be present in 0.6% of reverse-transcriptase genes [124] .

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