Antibody Engineering for Pursuing a Healthier Future

Saeed, Abdullah F. U. H.; Wang, Rongzhi; Ling, Sumei; Wang, Shihua

Flow cytometry (FC) is used to study antibodies on the cell surface and their related physiochemical properties. This technique was developed over 40 years ago (Robinson et al., 2012) , and allows the rapid detection of various proteins on cells. Proteins produce fluorescence or scattered light when passed through the machine sensing point to generate quantitative data on a large number of cells. Labeled antibodies are used for the detection of target protein molecules or antigens on the surface of cells (Álvarez-Barrientos et al., 2000) . FC has been used for the visualization of pulmonary clearance (Zhou et al., 2015) , fluorescence decay lifetimes, control sorting (Houston et al., 2010) , quantification of DNA-end resection in mammalian cells (Forment et al., 2012) , microsphere-based protease assays, highthroughput screening (Saunders et al., 2010a) , and botulinum neurotoxin type A light chain protease inhibitors (Saunders et al., 2010b) .