Antibody Engineering for Pursuing a Healthier Future

Saeed, Abdullah F. U. H.; Wang, Rongzhi; Ling, Sumei; Wang, Shihua

Phage display is a powerful biological technique for screening specific peptides or proteins. Screening of antibody libraries by phage display permits the rapid selection of scFvs to isolate V H and V L chains for mAb transformation. Thus, therapeutic antibodies against noxious or highly conserved antigens, plasma membrane proteins, and receptors can be obtained in their native conformation while avoiding animal immunization (Rader and Barbas, 1997) . The peptide libraries are incubated on a plate coated with the antigen of interest. Next, unbound phages are washed away. Then, the phages are amplified using host bacteria for high-affinity elution and binding/amplification cycles to expand the pool in favor of binding sequences. Finally, individual clones are characterized after 3-5 rounds by DNA sequencing and ELISA to achieve the resultant structural and functional details (Catanese et al., 2007) .