Author: Elsie Yekwa; Chutima Aphibanthammakit; Xavier Carnec; Bruno Coutard; Caroline Picard; Bruno Canard; Sylvain Baize; François Ferron
Title: Arenaviridae exoribonuclease presents genomic RNA edition capacity Document date: 2019_2_8
ID: fvp45ho6_15
Snippet: Results are expressed in Focus Forming Unit per mL (FFU/mL). For RNA quantification, viral RNA were extracted from cell culture supernatants (Qiagen, Courtaboeuf, France) and quantification was performed with the EuroBioGreen qPCR Mix Lo-ROX (Eurobio, Les Ulis, France), using an in house developed assay with 5'-CTTTCCCCTGGCGTGTCA-3' and 5'-GAATTTTGAAGGCTGCCTTGA-3' primers. Deep sequencing analysis of viral genomes was performed as described in [4.....
Document: Results are expressed in Focus Forming Unit per mL (FFU/mL). For RNA quantification, viral RNA were extracted from cell culture supernatants (Qiagen, Courtaboeuf, France) and quantification was performed with the EuroBioGreen qPCR Mix Lo-ROX (Eurobio, Les Ulis, France), using an in house developed assay with 5'-CTTTCCCCTGGCGTGTCA-3' and 5'-GAATTTTGAAGGCTGCCTTGA-3' primers. Deep sequencing analysis of viral genomes was performed as described in [44] .
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