Author: Yu, Liping; Zhang, Xiaorong; Wu, Tianqi; Su, Jin; Wang, Yuyang; Wang, Yuexin; Ruan, Baoyang; Niu, Xiaosai; Wu, Yantao
Title: Avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (MDA5) signaling pathway by cleavage of the adaptor protein MAVS Document date: 2017_11_13
ID: 0zn1sqj9_17
Snippet: Eleven-day-old SPF chicken embryos were inoculated with IBV at 10 3 EID 50 via allantoic cavity. Three embryos inoculated with PBS served as a negative control. Three embryos from each group were killed at 72 h postinoculation to determine chMDA5, chIFN-β, chIFN-λ and antiviral protein chMx transcription levels in the trachea, lung, liver, kidney, muscle and intestine of embryos, with all tissues being immediately processed for RNA extraction......
Document: Eleven-day-old SPF chicken embryos were inoculated with IBV at 10 3 EID 50 via allantoic cavity. Three embryos inoculated with PBS served as a negative control. Three embryos from each group were killed at 72 h postinoculation to determine chMDA5, chIFN-β, chIFN-λ and antiviral protein chMx transcription levels in the trachea, lung, liver, kidney, muscle and intestine of embryos, with all tissues being immediately processed for RNA extraction.
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